RNAdvance Cell v2 - 真核细胞总 RNA 提取与纯化试剂盒

RNAdvance Cell v2 试剂盒

无需抽滤或离心,即可可靠地实现细胞RNA提取。

  • 持续稳定地提供高产量的纯核酸
  • 为 NGS 和其他基于 PCR 的应用提取高完整性 RNA
  • 适用于手动或自动处理操作

Explore From Cells Models

RNAdvance Cells 试剂盒工作流程视图

Genomics RNAdvance Cell v2 Workflow

试剂盒特点

概述
RNAdvance Cell v2 试剂盒可从培养细胞中分离出总 RNA。
 

高质量 RNA 提取
RNAdvance Cell v2 试剂盒可纯化获得高 RIN 分数的 RNA,增强下游应用的适用性。
 

灵活且规模可调整
将 RNAdvance  Blood 试剂盒与我们的 Biomek 工作站相结合,可提供高通量和相对较少的动手操作解决方案:两个 96 孔样本板可在大约 2 小时 15 分钟内完成纯化。
 

引文

Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113

  • Used to extract RNA from ESC and EpiSC cell lines

Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025

  • Used to extract RNA from MCF-7 cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Automated Demonstrated Methods for Biomek i-Series Workstations (PDF) Learn more about automated NGS Library Construction and Nucleic Acid Sample Preparation methods for Biomek i-Series workstations
Simultaneous DNA & RNA Extraction from Cultured Cells without Splitting Lysate We developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same cultured cell sample. The method utilizes a proprietary buffer to selectively bind RNA and DNA
Isolation of RNA from Bacteria using RNAdvance Tissue Kit This protocol optimizes RNA isolation from a wide range of bacteria using RNAdvance Tissues with lysozyme
FormaPure RNA: Extended Protocol for RNA Isolation from FFPE Sample This document provides instructions for using the FormaPure RNA extraction and purification kit.

技术文件

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