Analytical Ultracentrifugation (AUC) Webinar Gallery
Ready for a deeper understanding of AUC? No problem. Check out these webinars to get valuable insights into the advantages of AUC. You can also read more about AUC on our resources page.
Three Distinct Analytical Ultracentrifugation Methods for Virus and Viral Vector Characterizations
Prof. Susumu Uchiyama
Department of Biotechnology, Osaka University
July 2023
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is considered the gold standard method for the size distribution analysis of viral vectors for gene therapy, but you have other orthogonal AUC methods to choose from too, including band sedimentation AUC (BS-AUC) and density gradient equilibrium AUC (DGE-AUC). In this 33-minute, on-demand webinar, Prof. Dr. Susumu Uchiyama from Osaka University explains the principle of each method, provides examples of data analysis, and discusses the relationship of the results from the three methods.
The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
Lake Paul, PhD
BioAnalysis, LLC
September 2020
As SV-AUC becomes the go-to method for quantitation and characterization of AAV, caution must be taken during the experimental design and analysis. Dr. Paul presents approaches to correctly analyzing AAV systems, explains how to apply AUC in a cGMP environment and how to develop proper experimental design for AAV systems and extracting the correct information.
Measuring Molecular Interactions by Multi-Wavelength AUC
Borries Demeler, PhD
University of Lethbridge, Alberta
June 2020
In this webinar. Dr. Demeler covers situations commonly encountered in the characterization of biomolecular interactions using AUC and shares his experience performing multi-wavelength analysis with the Optima AUC.
Looking under the Hood and Getting Started with the Optima AUC
Akash Bhattacharya, PhD
Beckman Coulter Life Sciences
October 2019
This webinar explores the operational aspects of AUC, including the technical advancements of the Optima AUC and the difference between absorbance and interference mode detection. It also covers designing a new experiment, transferring the data, and using simulation tools to model scans for a two-analyte system.
An Intro to Characterization of Biomolecules Using AUC
Akash Bhattacharya, PhD
Beckman Coulter Life Sciences
June 2019
Dr. Bhattacharya covers the principles of AUC, the fundamentals of how to design and set up experiments, the different types of AUC experiments, and the basic principles behind AUC data analysis. In addition, he shares a few sample applications.
Analytical Methods to Measure Empty and Full AAV Particles
Christine Le Bec, PhD
Genethon
June 2017
In this webinar, Dr. Le Bec discusses the proof of concept and qualification method of characterizing empty and full AAV particles with analytical ultracentrifugation.
A New Dimension in Simplified AUC Analysis
Borries Demeler, PhD
University of Texas, San Antonio
2017
Dr. Demeler is the creator of Ultrascan, an AUC software analysis program. In this video, he talks about developing software that optimizes data analysis from multi-wavelength experiments that are possible with the Optima AUC.
AUC for Structural Analysis - Proteins and Macromolecular Complexes
Andrew Herr, PhD
Cincinnati Children's Hospital
August 2015
In this webinar, Dr. Herr provides an overview of Analytical Ultracentrifugation (AUC) as a complementary technique for structural analysis of proteins and macromolecular complexes. He also highlights some ways AUC can be used specifically by structural biologists, but also by people doing protein chemistry, biochemistry, pharmaceuticals, and in biotechnology.
Advances in Sedimentation Analysis
Borries Demeler, PhD
University of Texas, San Antonio
April 2015
Dr. Demeler covers advances in sedimentation velocity (SV) analysis including methods for accurately characterizing heterogeneous systems at high resolution; approaches to extract rich information from SV experiments and techniques to measure the strength of macromolecular interactions with discrete binding models to whole boundary SV experiments in AUC.
Analysis of Particle Size Distributions by Analytical Ultracentrifugation
Helmut Cölfen, PhD
University Konstanz
2015
In this webinar, Dr. Cölfen discusses some of the principles of AUC and later presents his experience with the analysis of particle size distributions by using data from analytical ultracentrifugation.
Analytical Ultracentrifugation of Carbon Nanotubes
Jeffrey Fagan, PhD
National Institute of Standards and Technology
November 2014
Sedimentation velocity (SV) experiments on dispersed nanoparticles present an effective means for determining the hard-to-evaluate properties of nanoparticles. In this webinar, Dr. Fagan focuses on leveraging the capabilities of SV methods for characterizing single-wall carbon nanotubes (SWCNTs).
Aggregation Analysis and Beyond: AUC in the Biopharmaceutical Industry
Dr. Alexander Bepperling
Sandoz AG
October 2014
In this webinar, Dr. Bepperling explains how to set up continuous c(S) distribution analysis as implemented in the software SEDFIT in order to achieve enhanced reproducibility during a study of many batches of one protein. He will also share some applications of sedimentation equilibrium (SE) for the screening of weak interactions and the determination of detergent binding to proteins.
