Microfluidic Module

The microfluidics (MF) module for the BioLector XT microbioreactor allows you to run up to 32 highly flexible, pH-controlled fed-batch cultivations in microscale during one cultivation experiment run.

The ready-to-use MF microtiter plate (MTP) is filled and sealed with a foil inside a biosafety cabinet. Afterwards, the BioLector XT gassing lid is placed atop the plate, sealing the MTP against potential risks of contamination. The plate-lid combination is now protected from the outer environment and can be carried to the BioLector instrument, maintaining sterility inside.

After placing the plate-lid combination inside the microbioreactor, both are connected via gas tubing. During cultivation, the feed and pH control are maintained and controlled well-specifically for every individual cultivation. The new BioLection software allows for easy and user-friendly setup of process-oriented fed-batch profiles, that can be freely combined as necessary.

Independent of the MF module all standard features of the microbioreactor remain unchanged. Additionally, the instrument can be equipped with any or all other BioLector XT gassing modules, all of which can be used in combination with the MF module.

Anaerobic Cultivation

The anaerobic module for the BioLector XT microbioreactor enables strict anaerobic fermentation processes combined with a controlled, low nitrogen gas flow rate. This enables you to run cultivations under the exclusion of oxygen without the need of placing the instrument itself in an artificial atmosphere.

The microtiter plate (MTP) is filled and sealed with a foil inside an anaerobic bench. Afterwards, the BioLector XT gassing lid is placed atop the plate, sealing the MTP tightly against incoming gas. The plate-lid combination is now protected from the outer environment and can be carried to the BioLector instrument, maintaining the anaerobic conditions inside.

After placing the plate-lid combination inside the microbioreactor, both are connected via gas tubing. During fermentation, a continuous flow of nitrogen through the MTP-lid headspace is maintained. Therefore, any exposure to oxygen is prevented, providing a constant anaerobic environment.

Independent of the anaerobic module all standard features of the microbioreactor remain unchanged. In addition, the instrument can be equipped with any or all other BioLector XT modules at the same time.

CO2 Up Regulation

With the CO₂ up-regulation module the microbioreactor continuously measures the CO₂ level inside the chamber and automatically regulates the flow of CO₂ into the chamber.

For cultivations under CO₂-controlled conditions the BioLector microbioreactor system is equipped with a special CO₂ up-regulation module. This module consists of an additional gas connection for CO₂ on the back side of the BioLector and a CO₂ sensor inside the cultivation chamber.

The sensor continuously measures the CO₂ level inside the chamber and automatically regulates the flow of CO₂ into the chamber. In this way, a CO₂ level between 0 % and 10 % can be generated in the chamber. Independent of the additional module, all of the microbioreactor's standard features remain unchanged.

Up to three additional fluorescence modules can be added to the standard filters for biomass, pH, and DO. In total, six different modules fit into the system.

LED Filter

With the LED / filter module, the microbioreactor can measure additional fluorescences. An LED unit and two optical filter glasses are installed inside the BioLector microbioreactor.

For the measurement of emission and excitation wavelength, the BioLector microbioreactor system is equipped with a special LED / filter module. The wavelengths are freely selectable between 365 nm and 800 nm.
A new generation of low pH optodes measure the pH value in a range of 4-6.
Independent of the additional module, all of the microbioreactor's standard features remain unchanged. 

Up to three additional fluorescence modules can be added to the standard filters for biomass, pH, and DO. In total, six different modules fit into the system.

O2 Down Regulation

With the O₂ down-regulation module the microbioreactor continuously measures the oxygen level inside the chamber and automatically regulates the flow of nitrogen into the chamber.

For fermentation under microaerophilic conditions, the BioLector microbioreactor system is equipped with a special O₂ down-regulation module. It consists of an additional gas connection for nitrogen on the backside of the BioLector and an oxygen sensor inside the cultivation chamber.

The sensor continuously measures the oxygen level inside the chamber and automatically regulates the flow of nitrogen into the chamber. In this way, an oxygen level between 2 % and 21 % can be generated in the chamber.

Independent of the additional module, all standard features of the microbioreactor remain unchanged.

Up to three additional fluorescence modules can be added to the standard filters for biomass, pH, and DO. In total, six different modules fit into the system.

O2 Up Regulation

With the O₂ up-regulation module the microbioreactor continuously measures the oxygen level inside the chamber and automatically regulates the flow of oxygen into the chamber.

For fermentation with O₂-enriched air, the BioLector system is equipped with a special O₂ up-regulation module. It consists of an additional gas connection for oxygen on the back side of the BioLector, as well as an oxygen sensor inside the cultivation chamber.

The sensor continuously measures the oxygen level inside the chamber and automatically regulates the flow of oxygen into the chamber. In this way, an oxygen level between 21% and 35% can be generated in the chamber.

Independent of the additional module all standard features of the microbioreactor remain unchanged. 

Up to three additional fluorescence modules can be added to the standard filters for biomass, pH, and DO. In total, six different modules fit into the system.