DURAClone IF T Cell Activation
Identification of cytokine secreting T cell subsets is an important tool in studying and understanding T cell function in immune activation and suppression. The DURAClone T Activation panel assesses inflammatory cytokines in CD4+ and CD8+ T cell subsets at the single cell level. It utilizes dry reagent technology that confers workflow improvements.
- Panel of 6 markers in fluorochrome combinations that provide robust population identification, including CD3, CD4, CD8, IL-2, IFN gamma, TNF alpha
- Pre-formulated antibody panel in a unitized assay eliminating antibody cocktailing
- 25 tests per kit including 3 compensation kits with lot matched tandem dyes
For Research Use Only. Not for use in diagnostic procedures.
Features
Expert Panels
- Proven marker combination assessing pro-inflammatory cytokine expression in CD4+ and CD8+ T cells
- Open channels to add drop-in markers to modify for different research questions
- Designed for assay precision and lot-to-lot consistency
Efficiency
- Eliminate time spent in titrating different antibody lots
- Avoid waste that comes from preparing cocktail master mix
- Simplify inventory management
Consistency
- Unitized assays eliminate error prone pipetting steps
- Includes lot matched tandem dyes for compensation controls for improved accuracy
- Dry format maintains reagent performance better than liquid under stress conditions
Easy to Use
- Ready to use assay with verified panel design
- Includes sample preparation and staining procedure
- Streamlined workflow, simultaneously stain cell surface and intracellular targets
Explore DURAClone IF T Activation Models
Technical Documents
Didn't find what you are looking for? For more results click
here.
Related Parts
DURAClone IM Count Tube, 25 Tests, RUO
The DURAClone IM Count is used for enumeration of CD45+ cells in whole blood while simultaneously differentiating live and dead cells.
ViaKrome 808 Fixable Viability Dye
The ViaKrome 808 Fixable Viability Dye is a thiol reactive fluorescent dye that covalently binds free thiol present on cellular proteins at a neutral pH.
IOTest 3 Fixative Solution, 100-200 Tests, RUO
The IOTest3 Fixative Solution allows whole blood or bone marrow specimen preparation by fixing the cell suspension after erythrolysis during immunophenotyping procedures. At least one wash is required between the lysis and the fixation steps. The IOTest3 Fixative Solution is optimized to work in combination with the IOTest3 Lysing Solution. IOTest Fixative Solution is a formaldehyde-based fixative solution designed for flow cytometry. This reagent is provided as a concentrated solution in PBS.
The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation.
Accurate detection of both intracellular and extracellular epitopes are obtained, while:
• There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional).
• Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step.
• Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
• Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.
