DURAClone IF T Helper Cell
Activated T helper cells differentiate into functionally diverse subsets with specific cytokine expression, depending on the context of the stimuli. The DURAClone IF T Helper panel assess signature cytokines of CD4+ T helper cell subsets. It utilizes dry reagent technology that confers workflow improvements.
- Panel of 5 markers in fluorochrome combinations that provide robust population identification, including CD3, CD4, IL-4, IL-17A, IFN gamma
- Pre-formulated antibody panel in a unitized assay eliminating antibody cocktailing
- 25 tests per kit including 3 compensation kits with lot matched tandem dyes
For Research Use Only. Not for use in diagnostic procedures.
Features
Expert Panels
- Proven marker combination for the assessment of signature cytokines delineating functionally distinct T helper cell subtypes
- Open channels to add drop-in markers to modify for different research questions
- Designed for assay precision and lot-to-lot consistency
Efficiency
- Eliminate time spent in titrating different antibody lots
- Avoid waste that comes from preparing cocktail master mix
- Simplify inventory management
Consistency
- Unitized assays eliminate error prone pipetting steps
- Includes lot matched tandem dyes for compensation controls for improved accuracy
- Dry format maintains reagent performance better than liquid under stress conditions
Easy to Use
- Ready to use assay with verified panel design
- Includes sample preparation and staining procedure
- Streamlined workflow, simultaneously stain cell surface and intracellular targets
Explore DURAClone IF T Helper Models
Technical Documents
Didn't find what you are looking for? For more results click
here.
Related Parts
The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation.
Accurate detection of both intracellular and extracellular epitopes are obtained, while:
• There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional).
• Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step.
• Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
• Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.
