ViaKrome Fixable Viability Dyes
The ability to stain dead cells with a viability dye and preserve that staining pattern after fixation is critical for many flow cytometry applications. Exclusion of the dead cells from the data allows cleaner separation and identification of live cell populations. The ViaKrome Fixable Viability product line includes dyes excited by most common lasers and emit in common channels to provide researchers flexibility in panel design.
- Includes dyes excited by common lasers such as 405 nm, 561 nm, 638 nm, and 808 nm lasers
- Soluble in the biologically compatible buffer, phosphate buffered saline (PBS), no DMSO required
- Improved reagent stability, decreasing the precautions required for reagents susceptible to hydrolysis, no desiccation required
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ViaKrome Fixable Viability Dye Features
Performance
- Improved performance over standard NHS ester-based dyes
- Staining conditions recommended to provide easy discrimination of dead cells
- ViaKrome dyes do not interfere significantly with signals from positive markers in multicolor panels
Versatility
- Compatible with surface and intracellular staining
- Range of dyes excitable by common lasers, provides flexibility for panel design
- Includes 808 nm excitable dye freeing up the remaining visible spectrum for cell identification or experimental markers
Consistency
- Optimized staining concentration and incubation time tested on both cell culture and PBMC samples
- QC criteria established with multiple lots and operators
- Robust assay performance, less critical staining parameters
Easy to Use
- Soluble in biologically compatible buffer, PBS
- Does not require organic solvents such as DMSO for reconstitution
- Desiccation during storage not required to prevent water vapor contamination
Explore ViaKrome Fixable Viability Dyes Models
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