SPRISelect Size Selection Performance and Data
SPRISelect Size Selection Solution Performance & Data
Next Generation Sequencing (NGS) is the most pervasive technology in genomics research today. The long list of front-end applications has varied library preparation requirements that demand flexible solutions. The size of DNA has a direct impact on generating desired sequencing results. The SPRISelect Reagent Kit is designed to give scientists more flexibility and control over the size selection process. With proven, industry leading Solid Phase Reversible Immobilization (SPRI) technology, this reagent provides robust, reproducible and customizable size selection.
- Tunable from 150 to 800 base pairs allows for easy adjustments for specific applications and sequencers
- Efficient mag bead technology eliminates the need for gels, chips or special instrumentation
- Scalable from manual to automated for high throughput processing in 96-well plates
Dual Size Selection Capability
Left size selection removes DNA that is smaller than the targeted size, and right size selection removed DNA that is larger than the target size. By utilizing both left and right size selection you can target a smaller range of DNA sizes.
SPRISelect was used for dual size selection on sheared gDNA from E. coli. [Figure A] Left size selection with a ratio for 400bp. [Figure B] Right size selection with a ratio for 200bp. [Figure C] An overlay of the left size selection (red) and right size selection (blue). [Figure D] Double size selection excludes fragments above and below target cutoffs and captures a targeted regions between 200bp and 400bp. [Figure E] Sheared gDNA from E. coli. For all DNA size was measured using an Agilent 2100 Bioanalyzer high sensitivity chip (Agilent). The peaks at 35 and 10300 are internal markers.
Reproducible and Tunable
SPRISelect is manufactured to tight specifications ensuring run to run and lot to lot reproducibility.
Two different size selections were performed on sheared gDNA from E. coli using SPRISelect. Triplicates were performed per size selection. Each of the triplicates show very consistent size selection capabilities. (Left) Bind to sample ratio of 0.5x for left side size selection (Right) Bind to sample ratio of 0.5x for right side size selection. DNA size was measured using an Agilent 2100 Bioanalyzer high sensitivity chip (Agilent). The peaks at 43 and 115 are internal markers.
(Left) Sizes election ratio of 0.5x. (Right) Size selection ratio of 0.5x.
Less Hands-On Time Than Other Suppliers
SPRISelect and two other commercially available kits were used for size selection on sheared gDNA from E. coli. (Left) The graph represents the time for single size selection or clean up. The SPRISelect workflow for a single size selection is 4.2 and 2 times faster than supplier A and supplier B, respectively. (Right) The graph represents the time for dual size selection. The SPRISelect workflow for dual size selection is 3.8 and 2 times faster than supplier A and supplier B, respectively. SPRISelect has the lowest processing time out of all three suppliers by at least 50%. The times were based on performing size selection for 8 samples manually.
SPRIselect Left Workflow
Selecting for DNA larger than a target size.
- Bind DNA to magnetic beads
- Separate beads from contaminants
- Wash magnetic beads with 85% ethanol to remove contaminants
- Elute DNA from magnetic beads
- Transfer to a storage plate
SPRIselect Right Workflow
Selecting for DNA smaller than a target size or selecting for DNA within a target region.
- Bind DNA to magnetic beads
- Transfer supernatant to a new plate
- Bind DNA to magnetic beads
- Separate beads from contaminants
- Wash magnetic beads with 85% ethanol to remove contaminants
- Elute DNA from magnetic beads
- Transfer to a storage plate
Simple to Run Manually or Automated
Estimated hands-on time and total time in minutes, required to perform dual size selection on 8, 24, 48 and 96 DNA samples using SPRISelect. The different methods can be performed either manually or automated on a Biomek workstation. The table represents automation times performed on a Biomek i7 hybrid. Difference in time between manual and automation is indicated. NR=Not Recommended.
| SPRISelect | Manual | Automated | ||
|---|---|---|---|---|
| Batch Size | 8 | Hands-on Time | 10 | 10 |
| Total Time | 20 | 40 | ||
| 24 | Hands-on Time | 15 | 10 | |
| Total Time | 25 | 42 | ||
| 48 | Hands-on Time | NR | 15 | |
| Total Time | NR | 51 | ||
| 96 | Hands-on Time | NR | 15 | |
| Total Time | NR | 60 | ||
Products for Size Selection
| PART # | NAME | PREPS |
|---|---|---|
| B23317 | SPRISelect Reagent | 5 mL |
| B23318 | SPRISelect Reagent | 60 mL |
| B23319 | SPRISelect Reagent | 450 mL |
