DNAdvance Performance and Data
Genomic DNA Extraction from Tissue Samples
The DNAdvance kit is a genomic DNA (gDNA) extraction reagent kit built on SPRI paramagnetic bead-based technology. It enables the purification of high quality DNA from tissue samples. The DNAdvance kit offers greater recovery of high quality gDNA than other commercially available DNA extraction kits. The extraction can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling genomic workstations.
- Compatible with PCR based downstream applications
- Over 3 times the yield vs other suppliers’ extraction chemistries
- Manual and automated workflows available
High Recovery of High-Quality gDNA
Genomic DNA was extracted from 20 mg liver tissue using DNAdvance and other suppliers’ kits. [Figure 1] Samples were quantified using the NanoDrop (Thermo Fisher Scientific). The DNAdvance kit recovered higher amounts of gDNA than the kits from the other suppliers. [Figure 2] Samples were accessed for purity using the NanoDrop (Thermo Fisher Scientific). Error bars represent the standard deviation of three technical replicates.
High Quality High Molecular Weight gDNA
DNAdvance isolates high quality gDNA. Genomic DNA isolated from 4 different tissue types using DNAdvance were run on the Agilent Genomic DNA Screen Tape to assess quality. DIN values from samples isolated using DNAdvance were of high quality for all tissue types. Sample electrograph traces of DNA extracted from tail (Figure 4) and kidney (Figure 5).
No Carryover Contaminants to Inhibit PCR Amplification or Downstream Applications
Figure 6: Ability to amplify DNA was assessed via qPCR using a primer set (forward primer 5´-ggacttcgagcaagagatgg-3´ and reverse primer 5´-agcactgtgttggcgtacag-3´) designed to span Exon 4 and 5 of the beta (β)-actin gene (ActB) to produce 327 base pair amplicons. The gDNA isolated from 4 tissue types isolated using the DNAdvance kit was amplifiable indicating that the kit removed PCR inhibitors.
DNA Extractions from a Variety of Tissue Types
The gDNA yield from five different tissue types. The yields were quantified using the NanoDrop (Thermo Fisher Scientific). The purity of samples was accessed using the NanoDrop (Thermo Fisher Scientific); the A260/280 ratios are shown in the last two columns and are satisfactory for downstream applications.
| Tissue Type | gDNA Tield (µG) | DNA Purity (A260/A280) |
|---|---|---|
| Liver | 33.5 | 1.9 |
| Brain | 8.07 | 1.8 |
| Kidney | 16.1 | 1.8 |
| Lung | 7.28 | 1.8 |
| Tail | 5.38 | 1.8 |
Visual Workflow
- Lyse tissue in Lysis Buffer and Proteinase K
- Bind DNA to magnetic beads
- Separate magnetic beads from contaminants
- Wash the magnetic beads with 70% ethanol to remove contaminants
- Repeat the wash step 3 times
- Elute DNA from magnetic beads
- Transfer to new plate
Extract DNA from Samples in Less Time with Less Pipette Movements
Automation Methods vs. Manual Timing
Estimated hands-on-time and total time in hours required to perform DNAdvance DNA extractions. The estimated time does not take into account the overnight lysis step. The chemistry can be performed either manually or automated on a Biomek Workstation. The table represents automated times preformed on a Biomek i-Series Multichannel with a 1200 µl head. The difference in time between manual and automation is indicated (NR=not recommended).
| DNAdvance | Manual | Automated | ||
|---|---|---|---|---|
| Batch Size | 8 | Hands-on-Time | 0.25 | 0.25 |
| Total Time | 0.50 | 0.87 | ||
| 24 | Hands-on-Time | 0.25 | 0.25 | |
| Total Time | 0.50 | 0.87 | ||
| 96 | Hands-on-Time | NR | 0.25 | |
| Total Time | NR | 0.87 | ||
| 480 | Hands-on-Time | NR | 0.25 | |
| Total Time | NR | 2.25 | ||
| 960 | Hands-on-Time | NR | 0.25 | |
| Total Time | NR | 4.25 | ||
DNAdvance Products for DNA Extraction from Tissue
DNAdvance reagent kit is available in two kit sizes based on your throughput needs. Click on the specific part number below for purchasing or request a quote. For more information check out our DNA isolation from tissue page featuring DNAdvance.
| PART # | NAME | PREPS |
|---|---|---|
| A48705 | DNAdvance Kit | 384 |
| A48706 | DNAdvance Kit | 9600 |
Not intended or validated for use in the diagnosis of disease or other conditions.
For Research Use Only. Not for use in diagnostic procedures.
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