Speed up your setup: The CytoFLEX Platform and how to fast-track 20-color panel design

The CytoFLEX LX is a new (6L) multi-laser instrument that allows the development of high content, multi-parameter flow cytometry applications. The integration of avalanche photodiodes (APDs) detectors, drastically increases the linearity range of signal detection- when compared to traditional photomultiplier tube (PMTs)- allowing the detection of very dim and very bright signals at the same time from lasers of standard power. In our hands, this discrimination and resolution has considerably eased the development of one 20 color panel to identify 33 populations of murine splenocytes. - H. Luche