Analytical Ultracentrifugation Publications

Year Title Journal Authors DOI
2022 Best Practices for Aggregate Quantitation of Antibody Therapeutics by Sedimentation Velocity Analytical Ultracentrifugation Journal of Pharmaceutical Sciences George M. Bou-Assaf, Ivan L. Budyak, Michael Brenowitz, Eric S. Day, David Hayes, John Hill, Ranajoy Majumdar, Paola Ringhieri, Peter Schuck, Jasper C. Lin doi.org/10.1016/j.xphs.2021.12.023
2022 Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions Analytical Biochemistry Amy Henrickson, Gary E. Gorbet, Alexey Savelyev, Minji Kim, Jason Hargreaves, Sarah K. Schultz, Ute Kothe, Borries Demeler doi.org/10.1016/j.ab.2022.114728
2021 Density Matching Multi-wavelength Analytical Ultracentrifugation to Measure Drug Loading of Lipid Nanoparticle Formulations ACS Nano Amy Henrickson, Jayesh A. Kulkarni, Josh Zaifman, Gary E. Gorbet, Pieter R. Cullis, Borries Demeler doi.org/10.1021/acsnano.0c10069
2021 Reincarnation of the Analytical Ultracentrifuge: Emerging Opportunities for Nanomedicine Analytical Chemistry Olenka Jibaja Valderrama and Ivo Nischang doi.org/10.1021/acs.analchem.1c03116
2021 Comprehensive Size Distribution and Composition Analysis of Adeno-Associated Virus Vector by Multiwavelength Sedimentation Velocity Analytical Ultracentrifugation Journal of Pharmaceutical Sciences Takahiro Maruno, Kaede Usami, Kentaro Ishii, Tetsuo Torisu, Susumu Uchiyama doi.org/10.1016/j.xphs.2021.06.031
2021 Analytical Ultracentrifugation for Analysis of Protein-Nucleic Acid Interactions Methods of Molecular Biology Andrea Bogutzki and Ute Curth doi.org/10.1007/978‑1‑0716‑1197‑5_19
2021 Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans Molecules Stephen E. Harding doi.org/10.3390/molecules26196080
2021 Boundary convection during sedimentation velocity in the Optima analytical ultracentrifuge Analytical Biochemistry Steven A. Berkowitz and Thomas Laue doi.org/10.1016/j.ab.2021.114306
2021 Analytical band centrifugation for the separation and quantification of empty and full AAV particles Molecular Therapy – Methods & Clinical Development Harshit Khasa, Greg Kilby, Xiaoyu Chen, Chunlei Wang doi.org/10.1016/j.omtm.2021.04.008
2020 Analytical ultracentrifuge: an ideal tool for characterization of non-coding RNAs European Biophysics Journal Maulik D. Badmalia, M. Quadir Siddiqui, Tyler Mrozowich, Darren L. Gemmill, Trushar R. Patel doi.org/10.1007/s00249‑020‑01470‑9
2020 High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2 Biochemical and Biophysical Research Communications Luca Zinzula, Jerome Basquin, Stefan Bohn, Florian Beck, Sven Klumpe, Günter Pfeifer, István Nagy, Andreas Bracher, F. Ulrich Hartl, Wolfgang Baumeister doi.org/10.1016/j.bbrc.2020.09.131
2020 Measuring aggregates, self-association, and weak interactions in concentrated therapeutic antibody solutions mAbs Sumit K. Chaturvedi, Arun Parupudi, Kristian Juul-Madsen, Ai Nguyen, Thomas Vorup-Jensen, Sonia Dragulin-Otto, Huaying Zhao, Reza Esfandiary, Peter Schuck doi.org/10.1080/19420862.2020.1810488
2020 Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment PLOS Computational Biology Alexey Savelyev, Gary E. Gorbet, Amy Henrickson, Borries Demeler doi.org/10.1371/journal.pcbi.1007942
2020 Analytical ultracentrifugation: still the gold standard that offers multiple solutions European Biophysics Journal Renwick C. J. Dobson and Trushar R. Patel doi.org/10.1007/s00249‑020‑01483‑4
2020 Quantitation of soluble aggregates by sedimentation velocity analytical ultracentrifugation using an optical alignment system – Aspects of method validation Analytical Biochemistry Brandon L. Doyle, Adam P. Rauk, William F. Weiss 4th, Ivan L. Budyak doi.org/10.1016/j.ab.2020.113837
2020 A comparison of data quality using quartz vs. sapphire cell windows in analytical ultracentrifugation European Biophysics Journal Akash Bhattacharya and Eric Von Seggern doi.org/10.1007/s00249‑020‑01454‑9
2019 Optimized filopodia formation requires myosin tail domain cooperation Proceedings of the National Academy of Sciences Ashley L. Arthur, Livia D. Songster, Helena Sirkia, Akash Bhattacharya, Carlos Kikuti, Fernanda Pires Borrega, Anne Houdusse, Margaret A. Titus doi.org/10.1073/pnas.1901527116
2018 Precise determination of protein extinction coefficients under native and denaturing conditions using SV-AUC European Biophysics Journal Andreas Hoffmann, Kerstin Grassl, Janine Gommert, Christian Schlesak, Alexander Bepperling doi.org/10.1007/s00249‑018‑1299‑x
2018 Multi-wavelength analytical ultracentrifugation of human serum albumin complexed with porphyrin European Biophysics Journal Courtney N. Johnson, Gary E. Gorbet, Heidi Ramsower, Julio Urquidi, Lorenzo Brancaleon, Borries Demeler doi.org/10.1007/s00249‑018‑1301‑7
2018 Analytical ultracentrifugation (AUC): a seminal tool offering multiple solutions European Biophysics Journal Olwyn Byron, Ivo Nischang, Trushar R. Patel doi.org/10.1007/s00249‑018‑1333‑z
2018 Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes Molecular Therapy – Methods & Clinical Development Shelley A. Nass, Maryellen A. Mattingly, Denise A. Woodcock, Brenda L. Burnham, Jeffrey A. Ardinger, Shayla E. Osmond, Amy M. Frederick, Abraham Scaria, Seng H. Cheng, Catherine R. O’Riordan doi.org/10.1016/j.omtm.2017.12.004
2015 Enrichment and characterization of ferritin for nanomaterial applications Nanotechnology Rodolfo Ghirlando, Radina Mutskova, Chad Schwartz doi.org/10.1088/0957‑4484/27/4/045102
2015 Characterization of Homogeneous, Cooperative Protein–DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy Methods in Enzymology Ingrid Tessmer and Michael G. Fried doi.org/10.1016/bs.mie.2015.06.036
2015 Analytical Ultracentrifugation and Its Role in Development and Research of Therapeutical Proteins Methods in Enzymology Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule, Steven J. Shire doi.org/10.1016/bs.mie.2015.04.008
2015 Analytical Ultracentrifugation as an Approach to Characterize Recombinant Adeno-Associated Viral Vectors Human Gene Therapy Methods Brenda Burnham, Shelley Nass, Elton Kong, MaryEllen Mattingly, Denise Woodcock, Antonius Song, Samuel Wadsworth, Seng H. Cheng, Abraham Scaria, Catherine R. O’Riordan doi.org/10.1089/hgtb.2015.048
2015 Sedimentation Velocity: A Classical Perspective Methods in Enzymology John J.Correia and Walter F. Stafford doi.org/10.1016/bs.mie.2015.06.042
2014 Characterization of Size, Anisotropy, and Density Heterogeneity of Nanoparticles by Sedimentation Velocity Analytical Chemistry Borries Demeler, Tich-Lam Nguyen, Gary E. Gorbet, Virgil Schirf, Emre H. Brookes, Paul Mulvaney, Ala’a O. El-Ballouli, Jun Pan, Osman M. Bakr, Aysha K. Demeler, Blanca I. Hernandez Uribe, Nabraj Bhattarai, Robert L. Whetten doi.org/10.1021/ac501722r
2013 Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation Current Protocols in Protein Science Huaying Zhao, Chad A. Brautigam, Rodolfo Ghirlando, Peter Schuck doi.org/10.1002/0471140864.ps2012s71
2011 Determination of nanoparticle size distribution together with density or molecular weight by 2D analytical ultracentrifugation Nature Communications Randy P. Carney, Jin Young Kim, Huifeng Qian, Rongchao Jin, Hakim Mehenni, Francesco Stellacci, Osman M. Bakr doi.org/10.1038/ncomms1338
2008 Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium Methods in Cell Biology James L. Cole, Jeffrey W. Lary, Thomas P. Moody, Thomas M. Laue doi.org/10.1016/S0091‑679X(07)84006‑4
2007 Monitoring the homogeneity of adenovirus preparations (a gene therapy delivery system) using analytical ultracentrifugation Analytical Biochemistry Steven A. Berkowitz and John S. Philo doi.org/10.1016/j.ab.2006.11.031
2006 Role of Analytical Ultracentrifugation in Assessing the Aggregation of Protein Biopharmaceuticals The AAPS Journal Steven A. Berkowitz doi.org/10.1208/aapsj080368
2000 Molecular weight determination of membrane proteins by sedimentation equilibrium at the sucrose or Nycodenz-adjusted density of the hydrated detergent micelle Biochimica et Biophysica Acta (BBA) - Biomembranes Ariel Lustig, Andreas Engel, Georgios Tsiotis, Ehud M. Landau, Werner Baschong doi.org/10.1016/s0005‑2736(99)00254‑0