自动化下一代测序样品制备方法

我们与客户和先进技术供应商合作，开发并提供最佳实验解决方案。我们广泛的 Biomek 自动化下一代测序样品制备方法组合在使用真实样品情况下被证实可以生成高质量实验数据，并囊括越来越多 Illumina 合格的下一代测序（NGS）方法。

查看下面的方法列表和支持应用程序概述文档，查找您当前在实验室中使用的试剂盒或您计划将来评估的试剂盒。

DEMONSTRATED METHOD
BIOMEK i7
HYBRID
BIOMEK i7/i5
MULTICHANNEL
BIOMEK i5
SPAN-8
BIOMEK FXP
HYBRID
BIOMEK FXP/NXP
MULTICHANNEL
BIOMEX NXP
SPAN-8
BIOMEK 4000
CANCER PANELS
Illumina AmpliSeq Cancer HotSpot Panel v2
 
Illumina AmpliSeq Cancer HotSpot Panel v3
 
Illumina AmpliSeq Focus Panel
 
Illumina TruSight Oncology 500 DNA/RNA
 
Illumina TruSight Oncology 500 HT
 
Illumina TruSight Cancer
 
Illumina TruSight Tumor 15
 
Illumina TruSight Tumor 170
 
 
Illumina TruSight Tumor 500
 
NEB NEBNext Direct Cancer HotSpot Panel
 
 
TruSight RNA Pan Cancer
 
DNA SEQUENCING
ATAC-Seq
 
Illumina AmpliSeq for Illumina NGS
 
Illumina DNA PCR-Free Prep
 
Illumina ForenSeq
 
Illumina Nextera DNA Flex Library Prep Kit
 
Illumina Nextera XT
 
 
Illumina TruSeq Custom Amplicon Low Input Kit
 
Illumina TruSeq DNA PCR-Free
 
 
 
 
Illumina TruSeq Nano DNA
 
 
 
 
NEB NEBNext Ultra DNA for Illumina NGS (ChIP-seq and HLA)
 
NEB NEBNext Ultra II DNA Kit for Illumina NGS
 
 
NEB NEBNext Ultra II FS DNA
 
NuGEN Ovation Ultralow Library System v2
 
NuGEN Ovation Rapid Library System (PCR-Free)
 
Roche KAPA HyperPrep Library Prep Kit for Illumina NGS
 
 
 
 
 
Roche KAPA HyperPlus Library Prep Kit for Illumina NGS
 
 
 
 
 
Swift Biosciences Accel - NGS 2S Plus DNA Library Kit for Illumina NGS
 
Takara SMARTer ThruPLEX DNA-seq
 
Twist Library Preparation Kit (Mechanical & enzymatic fragmentation)
 
 
HLA TYPING
GenDx NGSgo
 
 
Illumina TruSight HLA Sequencing Panel v2
 
Immucor MIA FORA Flex HLA
 
 
 
 
 
Omixon Holotype HLA X2
 
RNA SEQUENCING
Illumina AmpliSeq for Illumina NGS
 
Illumina RNA Prep with Enrichment
 
Illumina TruSeq RNA v2
 
Illumina TruSeq RNA Exome
 
 
Illumina TruSeq Stranded mRNA
 
 
Illumina TruSeq Stranded Total RNA
 
 
Lexogen QuantSeq 3' mRNA -Seq Library Prep Kit
 
NEBNext ARTIC SARS-CoV-2 FS Express
 
NEB NEBNext Small RNA Kit for Illumina NGS
 
NEB NEBNext Ultra II Directional RNA Library Kit for Illumina NGS
 
 
NuGEN Ovation Universal RNA-Seq
 
NuGEN Trio RNA-Seq
 
NuGEN Universal Plus mRNA-Seq
 
Roche KAPA mRNA HyperPrep Kit
 
Roche KAPA RNA HyperPrep Kit with RiboErase (HMR)
 
SINGLE-CELL SEQ
10X Genomics Single Cell 3’ kit- Library Preparation
 
NEB NEBNext Single Cell/ Low Input RNA Library Prep Kit
 
Takara SmartSeq HT
 
TARGET/EXOME CAPTURE
Agilent HaloPlex Target Enrichment - Ion Torrent
 
 
 
Agilent SureSelect XT
 
 
IDT xGen Hybridization and capture of DNA Libraries
 
Illumina Nextera DNA Exome Kit
 
Illumina Nextera Flex Enrichment
 
Illumina Nextera Rapid Capture
 
 
Illumina TruSeq Exome
 
 
NEBNext Direct Genotyping Solution
 
NuGEN Allegro Targeted Genotyping
 
Roche Nimblegen SeqCap EZ for Illumina NGS
 
Twist Fast Hybridzation and Wash
 
Twist Standard Hybridization and Wash
 

方法说明

 
经验证方法。表明该套方法是在按照供应商出版的样品制备试剂盒使用手册要求开发的。这些方法无论是在客户或供应商实验室（客户/供应商验证），还是在贝克曼库尔特生命科学实验室（贝克曼验证）中，均通过使用相关的样品进行测试，结果均满足供应商试剂盒的性能要求。贝克曼库尔特对这些方法的使用或性能不作任何声明。
 
Illumina 合格方法。表明Illumina 对使用 Biomek 自动化制备的文库进行分析，自动化构建的文库与手动构建的文库性能相当。此方法不是 Illumina 产品，并且 Illumina 不直接支持此产品。Illumina 对本产品不作任何陈述或保证。
 
方法由试剂盒供应商创建和提供。
 
TruSight 认证的方法: 这些方法由 Illumina 和自动化合作伙伴共同开发，经过 Illumina 进行验证测试，并在用户现场通过 alpha 测试。 方法由自动化合作伙伴提供安装支持，illumina FAS 将提供方法培训并提供一线支持。自动化合作伙伴也提供一线技术支持以确定客户的问题是由方法，自动化硬件，还是试剂盒导致。



Biomek Automated Workstation Stations and methods are not intended or validated for use in the diagnosis of disease or other conditions. In some cases, method data was generated on pre-production automated workstations.

The automated methods identified are demonstrated methods only and are not validated by Beckman Coulter. Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. The demonstrated methods are available through Beckman Coulter automation field applications team.

© 2021 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All additional trademarks used throughout this document are the property of their respective owners. 21.08.2280 AUTO

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