Adenoviruses (Ads) are double-stranded non-enveloped DNA viruses which are highly infectious but also highly immunogenic. This means that while transduction efficiency can be potentially very high, there is also a high risk that immune activation will eliminate viruses before they reach their target, or eliminate transduced cells prior to transgene activation and transcription.
1 In addition, most individuals are infected with at least one adenovirus serotype early in life, leading to widespread pre-immunity. This limits the efficacy of adenovirus vectors for therapeutic usage.
1 For a further look into relevant areas and viral vectors please visit our resource center
here.
References:
1. W.S.M. Wold and K. Toth, “Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy,”
Curr Gene Ther 13(6): 421-433, 2013.