贝克曼细胞计数和活率分析仪
自 1954 年最初的库尔特计数器诞生以来，我们已经建立了一套完整的新方法，囊括细胞计数、粒度测量、活率分析和细胞培养监测系统，从而为您在生命科学领域开辟了新的可能性。我们创建的每个解决方案都从您的角度出发，结合您在实验当中所需的仪器性能，准确性和工作流程效率等的要求。我们的仪器内置合理可靠的工程设计，因此您可以依靠仪器来满足实验室的需求。我们不断推出新的先进技术，以改善您的工作流程，至今构成已了一套完整全面的细胞计数器体系，其中包括我们的Vi-CELL 平台 和 Multisizer 4e 细胞计数及分析仪。
通过使用精密的业界先进科技，每种细胞计数仪器采用不同的方法来帮助您计数，测量粒径和监控细胞的健康。细胞计数和分析仪所具备的功能全面且强大，同时依然能够实现直接简易的操作。因此，无论您是在寻求实验室全自动化，深入的实验结果，还是只是业内的高度可靠性，您都可以从这里找到满足您需求的解决方案。
FAQ on Cell Counters, Sizers and Media Analyzers
What factors can influence cell viability measurements?
Cell viability results can be affected by sample handling, incubation conditions, reagent selection, timing of analysis, and cell type. Standardized protocols help improve consistency and ensure reliable viability assessments across experiments.
What is a Coulter Counter?
A Coulter Counter counts and sizes particles suspended in an electrically conductive liquid by measuring changes in electrical impedance as individual particles pass through an aperture. Each detected pulse represents a particle event, while pulse amplitude is proportional to the volume of electrolyte displaced by the particle. When the analyzed sample volume is known, the measurements can also be used to determine particle concentration. The Multisizer 4e Coulter Counter applies this method to individual-particle counting and sizing.
The Coulter Principle is used to count and size particles suspended in an electrically conductive liquid. As particles pass individually through an aperture between two electrodes, each particle displaces electrolyte and produces a temporary change in electrical impedance. The number of pulses corresponds to the number of detected particles, and the amplitude of each pulse is proportional to particle volume. These data can be used to calculate particle size distribution and concentration.
When should a Coulter Counter be used instead of flow cytometry?
A Coulter Counter is appropriate when the primary objectives are to determine individual-particle count, concentration and volume-based size distribution without relying on fluorescent labels or particle optical properties. Samples must be suspended in an electrically conductive liquid and matched with an appropriate aperture.
Flow cytometry may be more appropriate when the study requires fluorescence-based identification, biomarker expression data or differentiation of populations using multiple optical parameters. Method selection should be based on the required information, sample characteristics and analytical workflow.
