Paper digest: Tracking CAR-T cells after infusion

The Paper Digest series highlights outstanding publications demonstrating the advanced use of flow cytometry in clinical research. Here you will find core elements from the work of Blache et al., Front Immunol. 2021, describing a standardized, yet flexibly adaptable framework for identification and comprehensive characterization of CAR-T cells post infusion.

Methodical focus refers to pre-analytical and analytical cytometry process phases.

Cytometry process phases

Flow cytometry process phases diagram

Pre-formulated dry antibody assays for flow cytometry

Flow cytometry sample staining workflow with pre-formulated dry reagents

Benefit

  • Reduction of technical error
  • Expert antibody panel design
  • Flexible panel adaptation
  • Fast turnaround time
  • Easy reagent storage

Comprehensive CAR-T Cell Phenotyping

Antibody panels for CAR-T immunophenotyping

Differential Activation/Exhaustion Marker Profiles Between CAR-/+ T Cells

Histogram LAG3 ECD differentiating between healthy control, CAR-, and CAR+ patient T-Cells
Histogram PD1 PC7 differentiating between healthy control, CAR-, and CAR+ patient T-Cells
Histogram TIM3 SN428 differentiating between healthy control, CAR-, and CAR+ patient T-Cells
Histogram CD69 PC7 differentiating between healthy control, CAR-, and CAR+ patient T-Cells
Histogram CD25 SN428 differentiating between healthy control, CAR-, and CAR+ patient T-Cells

Thin black linehealthy control CAR-

Thick yellow linepatient T-cells CAR-

Thick green linepatient T-cells CAR+

How this study was performed

Whole blood samples from CAR-T recipients and normal donors were stained with DURAClone dry reagents* IM Count, IM TCRs, RE ALB and LUCID custom dry reagents* together with liquid Drop-in reagents. Analyzed with Kaluza software*.

Clinical Trials

Talk to an expert

Talk to an expert

DURAClone Panels

LUCID Custom Design Services

Kaluza Analysis Software

Blache U, Weiss R, Boldt A, Kapinsky M, Blaudszun AR, Quaiser A, Pohl A, Miloud T, Burgaud M, Vucinic V, Platzbecker U, Sack U, Fricke S, Koehl U. Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications. Front Immunol. 2021 May 3;12:658314, reproduced pursuant to Creative Commons 4.0 Creative Commons Attribution 4.0 International License.

Access the full article

*For research use only. Not for use in diagnostic procedures.