血液 DNA 提取试剂盒
GenFind V3 试剂盒
GenFind V3 血液DNA提取试剂盒，采用基于固相载体可逆性固定法（SPRI）结合磁珠的技术，从全血中提取并纯化基因组 DNA（gDNA）。
- 一致的高纯度高质量的 gDNA 产物
- 无抗凝剂等抑制剂的残留，适用于 PCR/qPCR/ddPCR 和 NGS
Explore From Blood Models
GenFind V3 gDNA Extraction Workflow
- Lyse the sample
- Bind DNA to SPRI beads
- Separate beads from contaminants
- Wash beads to remove contaminants
- Elute DNA from beads
- Transfer to new plate
Data and Performance
High recovery of high molecular weight genomic DNA with GenFind V3 kit
Genomic DNA isolated from various blood collected in heparin, EDTA and citrate tubes was run on the Agilent Genomic DNA ScreenTape to assess quality. (Left) DIN values from all samples isolated with the GenFind V3 kit were all ≥ 9.0, indicating that high-quality and intact gDNAs were recovered. DNA isolated using Supplier A’s kit were of lower quality indicated with a DIN score of 7.8. (Right) Sample traces of the Genomic DNA isolated from tubes containing heparin.
Interested in more data? Explore the GenFind V3 data sheet.
Specifications
| Application | Genomic DNA extraction |
| Technology | SPRI paramagnetic bead-based technology |
| Sample types | Whole blood (fresh or frozen), serum, cultured cells, dry blood spots,* mouthwash,* saliva,* Gram-positive and Gram-negative bacteria (E. coli, S. aureus, Salmonella, Corynebacterium, Mycobacterium),* mucoid bacteria (P. aeruginosa),* fungi (A. niger),* lung tissue (fresh or frozen)* |
| Kit Components and Storage Conditions | Lysis bufer LBB (RT), Proteinase K (RT), Binding buffer BBB (4°C), Washing buffer WBB (RT), Washing buffer WBC (RT) |
| Input Volume Range | 50—400 μl |
| Processing mode | Automated or manual |
| Kit Sizes | 50 and 384 preps |
How to Automate GenFind V3 gDNA Extraction
When compared to manual operations, the GenFind V3 kit automated on Biomek liquid handlers provides:
- Reduced hands-on time and increased throughput
- Option to run the method end-to-end with only setup and tear-down touch points
- Reduction in pipetting errors
- Standardized workflow for improved results
- Quick implementation with demonstrated methods
To learn more about automation of the GenFind V3 gDNA extraction kit on the Biomek i7 Hybrid Genomics Workstation, read our application note.
To discuss the feasibility to automate the GenFind V3 gDNA extraction in your lab with one of our experts complete this form.
Citations
Nickels EM et al. (2022). DNA methylation at birth in monozygotic twins discordant for pediatric acute lymphoblastic leukemia. Nature Communications 13, 6077. doi: 10.1038/s41467-022-33677-z.
Tran NT et al. (2022). Precise CRISPR-Cas–mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells. Science Advances, 8(22), eabm9106. doi: 10.1126/sciadv.abm9106.
Tsuji T et al. (2020). Clonality and antigen-specific responses shape the prognostic effects of tumor-infiltrating T cells in ovarian cancer. Oncotarget, 11(27), 2669–2683. doi: 10.18632/oncotarget.27666.
Leung C et al. (2022). Chronic stress-driven glucocorticoid receptor activation programs key cell phenotypes and functional epigenomic patterns in human fibroblasts. iScience, 25(9), 104960. doi: 10.1016/j.isci.2022.104960.
技术文件
*Supplemental data from Beckman Coulter Life Sciences
†Customer-published data
Products and demonstrated applications are not intended or validated for use in diagnostic procedures.
Supplemental data and customer-published data references are provided for demonstration only, and are not validated by Beckman Coulter Life Sciences. Beckman Coulter Life Sciences makes no warranties express or implied with respect to this protocol, including warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. Your use of the method is solely at your own risk, without recourse to Beckman Coulter Life Sciences.
