蛋白质生产

Protein Production 在目前几种主要的蛋白质纯化方法中，仅有离心方法可以在无介质条件下对蛋白质进行纯化，保证蛋白质在纯化过程中保持其天然状态。这一点非常关键，因为蛋白质的构象和功能通常会在纯化过程中与膜或磁珠发生相互作用后而发生变化，从而改变纯化蛋白的目标特性。在无介质条件下，比如在高速离心机中, 不仅可以按分子量的差异性进行提纯，还可以按密度和构象的差异性进行提纯，而单一的膜纯化技术则无法同时提供这么多纯化方式。离心机可以提供蛋白样品纯化（必要时定量）所需的精确离心力，供研究或治疗工艺使用。

请浏览以下应用资源，回顾基础知识或完善您的工艺技术流程。

蛋白质生产相关产品

Avanti JXN-26系列高速离心机

• 支持 GMP 合规性要求
• 快速分离细胞
• 快速去除细胞碎片
• 保护最终产物

Optima 分析型超速离心机

• 质量表征
• 纯度一致性验证
• 更可靠的数据分析
• 实时数据分析

Optima XPN 系列超速离心机

• 支持 GMP 合规性要求
• 纯化高质量质粒，优化转染效果

无菌+无菌无酶离心管

• 保证高质量最终产品的一致性
• 优化工作流
• 提高数据质量

HarvestLine System Liners 样品收集袋

• 减少接触生物制剂的风险
• 最大限度降低样品污染风险
• 优化工作流

Vi-CELL BLU 细胞计数及活率分析仪

• 优化分析参数以提高准确性和与其他方法的关联性
• 24 位自动进样器支持免值守操作

CytoFLEX LX 流式细胞仪

• 使用特定生物标志物进行细胞定量或表征

*产品仅用于科研。不用于诊断。

Biomek 4000 实验室自动化工作站

• 精确追踪离心管液位
• 简洁明了的操作界面
• 保证样品组分的一致性

联系我们

Content and Resources

g-Max: Added Capabilities to Beckman Coulter's versatile Ultracentrifuge Line The g-Max System adds several valuable capabilities to your Beckman Coulter ultracentrifuge rotors:

Linearity of BSA Using Absorbance & Interference Optics The Optima AUC is the latest innovation from Beckman Coulter and offers significant advantages as an analytical ultracentrifugation platform over the predecessor ProteomeLab. Besides the plethora of advancements in user experience on the new platform, the Optima AUC’s optical, thermal, and drive systems have also been upgraded significantly, creating an overall better experience to the end-user in terms of data quality and ease of use.

Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation Insulin is a well characterized drug used to treat diabetes. A large number of insulin formulations have been investigated in order to develop the optimum time delivery suited to patient needs. In addition, a number of insulin analogs such as Insulin Glargine and LysPro insulin (Humalog) have been developed and marketed as derivatives for immediate use at meal time and a more sustained release.

Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin <p style="margin-right: 0px; margin-bottom: 0px; margin-left: 0px;">Analytical Ultracentrifugation (AUC) is the method of choice to characterize colloidal systems under physiological solution conditions.</p> <p style="margin-right: 0px; margin-bottom: 0px; margin-left: 0px;">Recently, a novel multi-wavelength (MWL) detector on Beckman Coulter’s Optima AUC has been developed, which for the first time, couples the hydrodynamic separation of colloidal mixtures to spectral deconvolution of interacting and non-interacting solutes present in a mixture.</p>

Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex Proteins have a variety of cellular functions, structures, and mechanisms of action. Routinely, proteins bind other biomolecules, or ligands, in order to complete a task. Researchers gain valuable knowledge on how proteins work in a cellular environment by purification of proteins bound to their appropriate substrate.