OptiMATE Gradient Maker

for Density Gradient Centrifugation Sample Preparation

The OptiMATE Gradient Maker simplifies the laborious and error-prone density gradient ultracentrifugation (DGUC) process into an easy-to-maintain operation by automating nearly all pre-centrifuge steps, including gradient preparation, accurate dispensing, and tube sealing, significantly reducing run times without sacrificing purity or yield.

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OptiMATE gradient maker for density gradient centrifugation
OptiMATE gradient maker for density gradient centrifugation

Features of the OptiMATE Gradient Maker

Automated dispense and tube sealing

  • Significantly reduce error and improve reproducibility
  • Compatible with most common Beckman Coulter tube sizes
  • Compatible with Quick-Seal, OptiSeal, and Open-Top tubes
     

Pre-formed gradients

  • Ability to dispense linear and step gradients
  • Dramatically shorter run times (75+% reduction) for linear gradients

Simple GUI and method creation

  • Train new operators in just hours, not weeks
  • Published methods ensure consistency between runs and operators
  • On-board visualization of gradient profiles

Introducing the OptiMATE Gradient Maker

 


Automated dispense and tube sealing

Pre-formed gradients

Simple GUI and method creation

Automated, Consistent, and Efficient Gradient Dispensing


  • Automated dispensing of both step and linear gradients, and automated tube sealing

  • Significantly shorter run times by starting with pre-formed linear gradients

  • Improved consistency – reduce errors with automation

  • Generate high-quality gradients in just hours even without density gradient proficiency

  • Compatible with cesium chloride (CsCl), iodixanol (IDX), and sucrose
OptiMATE Gradient Maker performance data

Resources

Rapid, Automated Purification of Adeno-Associated Virus using the OptiMATE Gradient Maker

 

Read

Reducing Variability and Hands-On time in Viral Vector purification using the OptiMATE Gradient Maker

 

Read

See Density Gradient Reagents

Explore OptiMATE Gradient Maker Models

Frequently Asked Questions

  • Cesium Chloride
  • Iodixanol (OptiPrep™)
  • Sucrose

Sizes
  • 16 mm (8.9 to 13.5 mL)
  • 25 mm (29.9 to 39 mL)
  • 38 mm (94 mL)
Formats
  • Quick-Seal
  • Opti-Seal
  • Open-Top
Materials
  • Polypropylene
  • Ultra-Clear
Filling and sealing performance are impacted by tube material, tube size, and tube volume. Not all tubes meeting the above criteria may be suitable. For a complete list of compatible rotors and tubes, please see the instrument brochure and instructions for use (IFU).

No, OptiMATE is only compatible with ultracentrifuge tubes provided by Beckman Coulter Life Sciences.
Yes, tubing lines are reusable. They have been tested to three runs.

Yes, tubing sets can be sterilized via autoclave or ethylene oxide (EtO).
Density gradient ultracentrifugation (DGUC) is a centrifuge-based technique for providing superior purification of viral vectors (e.g., isolating full AAV particles from partial and empty capsids), along with other materials (such as plasmid DNA) in gene therapy production workflows. DGUC results in a layered gradient based on the density of materials in the original sample.
  • Viruses / viral vectors (e.g., AAV)
  • Virus-like particles and bacteriophage
  • Extracellular vesicles (EVs)
  • Proteins / protein complexes
  • Lipid nanoparticles
  • Synthetic nanoparticles or other nanomaterials
  • Nucleic acids / plasmids
  • Lipemic separations

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技术文件

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