SuperNova Fluorescent Polymer Dyes

SuperNova v428

SuperNova v428 (SN v428), the first dye of the series, is optimally excited by the violet laser (405 nm) with an excitation maximum of 414 nm. It has an emission peak of 428 nm and can be detected using a 450/50 bandpass filter or equivalent. It is among the brightest dyes excitable by the violet laser, and is therefore particularly suited for the assessment of dimly expressed markers.

Figure 1. Absorption and emission spectra λexc = 405 nm of SuperNova v428 in PBS.

SuperNova v605

SuperNova v605 is a tandem polymer dye, derived from the core SN v428. Therefore both share the same absorbance characteristics, with a maximum excitation at 414 nm. With emission peaks at 605 nm, it is optimally detected using the 610/20 nm bandpass filters of the flow cytometer.

Figure 2. Absorption and emission spectra λexc = 405 nm of SuperNova v605 in PBS.

SuperNova v786

SuperNova v786 is a tandem polymer dye, derived from the core SN v428. Therefore both share the same absorbance characteristics, with a maximum excitation at 414 nm. With emission peaks at 786 nm, it is optimally detected using the 780/60 nm bandpass filters of the flow cytometer.

Figure 3. Absorption and emission spectra λexc = 405 nm of SuperNova v786 in PBS.

SuperNova tandem-dye technology

SN v428 is a Beckman proprietary bright polymer dye that can be activated with amine for tandem dyes, followed by maleimide activation for tandem conjugates. Rigidity of our polymer dye structure helps to reduce rotational energy leading to brighter emissions. This along with our exceptional manufacturing in proprietary formulations helps to achieve optimized FRET efficiency and increased stability.

Figure 4. SuperNova tandem dye technology.