Preparing a CytoFLEX for Nanoscale Flow Cytometry
George Brittain, Sergei Gulnik, and Yong Chen, Beckman Coulter Life Sciences, Miami, FL 33196Introduction
Built around semiconductor technology, with a number of innovations to enhance light capture, reduce noise, and prevent signal losses, the CytoFLEX is capable of detecting biological nanoparticles (NPs) as small as 80nm by light scatter, and has a linear fluorescence range that extends down into the single digits for fluorophores like FITC. However, in order to properly setup the CytoFLEX for NP analyses, a variety of considerations need to be taken into account.
In this poster, we will demonstrate how to properly setup and clean a CytoFLEX flow cytometer for NP analyses. First, we will explore the different threshold options and sensitivity ranges. Next, we will show how to setup violet side scatter (VSSC) triggering. And finally, we will discuss several important issues that affect proper sample analyses, including how to clean the instrument to reduce noise within the nanoparticle range.
Materials
Methods
- Upon startup, the instrument was primed, cleaned and flushed.
- The beads were mixed together and diluted with HPLC water.
- Extracellular Vesicles (EVs) were prepared from fresh human blood as follows:
- 2mL of K3-EDTA blood was first aliquotted into 12x75mm centrifuge tubes. Cells in larger volumes, further away from the max radius, do not pellet as well within a short time frame and larger EVs pellet with longer time frames, so if a larger volume is needed, increasing the number of tubes works better than a greater volume per tube.
- The blood was centrifuged for 5 min at 200xg to pellet the majority of cells and platelets.
- Roughly 1mL of platelet-poor plasma (PPP) was removed from the top, careful to minimize collection of the platelet-rich plasma near the WBC layer.
- The PPP was filtered through a 200nm syringe filter to remove residual large particles.
- Finally, the filtered PPP was further purified using Izon size-exclusion columns to remove particles smaller than 70nm.
- All samples were acquired on a CytoFLEX-S N-V-B-R, and the data were analyzed in CytExpert v2.3.
I. Light-Scatter Sensitivity
Forward Scatter (FSC) on the CytoFLEX is not smallangle scatter, as found on a typical flow cytometer. It is a digital signal-analysis method, called axial light loss detection, which is optimized for 500nm-50m particles. It depends directly on the particle volume and is mostly independent of the refractive index.
Side Scatter (SSC) is much more sensitive, but is attenuated for use with cells and other large particles. It is optimized for roughly 200nm- 20µm.
Violet SSC (VSSC) is a 3rd scatter-detection mode that is available to take full advantage of the CytoFLEX sensitivity. VSSC can fully resolve 80nm PS or 100nm Si beads.
II. Setting up VSSC-H Triggering
- Set the Configuration to VSSC
- If a VSSC configuration does not exist, start a new one.
- Pick a channel and assign it as VSSC with the 405/10 filter.
- Save the configuration and set it as Current.
- Physically Swap the Filters
- Make sure that there is a filter in each position preceding the channels of interest or the light path will be broken.
- Do not place an equivalent bandpass filter upstream of a channel of interest or this will also result in the loss of the desired signal.
- Set the Threshold to VSSC-H
- VSSC-Height is the most sensitive trigger on the CytoFLEX for lightscatter detection. VSSC triggering requires 1, 2, and 3 together.
- The specific threshold level will depend on the VSSC Gain, and will change proportionally to any adjustments to the gain.
- The instrument always triggers in Height. Area back-calculates a relative Height setting, so it is more precise to set the Height directly.
- Change the Event Rate Setting to High
- If the Event Rate Setting is in Default, change it to High.
- The Default setting broadens the pulse window in order to help with setting up laser alignments and delays if they are out of line. The broader window will also increase the optical noise sampled.
- The High setting reduces background and improves event processing. The instrument should always be set on High for sample acquisition.
III. Important Considerations for Effective Nanoscale-Flow-Cytometry Experimentation
- Identify the Optimal Threshold
- Use appropriate reference particles to identify the optimal threshold. Using buffer alone will not properly identify the detection boundaries.
- On the CytoFLEX, the appropriate threshold level is generally around 10-fold higher than the gain. Above, the Gain = 400, Threshold = 3K.
- Clean the Sample Probe
- Debris from samples and buffers builds up in the probe, mostly visible below the 100nm PS range. Carryover can even occur between reads.
- Clean the probe by running a panel of Bleach, FlowClean, and then 2x Water to flush, for 1-2 min each at the max rate. Repeat if needed.
- Use Clean Buffers
- Any particulate in the sample buffers will also be detected as events.
- Filtering dirty buffers is generally insufficient because the particulate is often smaller than the filter. Discard and use fresh clean buffers.
- Optimize the Sample Concentration
- Flow cytometers have an optimal rate, inversely proportional to size. If the sample concentration is too high, swarming will occur.
- The concentration needs to be titrated to find the optimal range.
- At the appropriate concentration range, different dilutions will result in a shift up or down on the Counts axis, without shifting the intensity.
- The Abort Rate is also a swarming indicator: <5% is best.
- The Abort Rate Can Help Optimize
- The Abort Rate is particularly helpful with optimizing the dilutions of complex samples, e.g., without a tight monomodal distribution.
- When under 10%, the population medians should be consistent between dilutions. <5% is best.
- Balance the Concentration and Rate
- The sample rate is an instrument-based dilution directly into the flow cell. Faster rates proportionally increase the effective concentration.
- Faster rates also broaden the core stream and can increase CVs.
Discussion
Ultimately, the CytoFLEX is one of the most sensitive flow cytometers on the market. However, with such great power comes great responsibility to properly prepare the instrument and samples for effective nanoscale-flow-cytometry experiments. Nanoscale flow cytometry introduces a number of additional variables that are largely extraordinary to conventional flow cytometry, and each requires careful attention.
The CytoFLEX is for Research Use Only. The Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All other trademarks are the property of their respective owners.
Helpful Links
-
阅读材料
-
应用手册
- 17-Marker, 18-Color Human Blood Phenotyping Made Easy with Flow Cytometry
- 21 CFR 第 11 部分关于在线 WFI 仪器的数据完整性要求
- 8011+ Reporting Standards Feature and Synopsis
- Achieving Compliant Batch Release – Sterile Parenteral Quality Control
- Air Particle Monitoring ISO 21501-4 Impact
- An Analytical Revolution: Introducing the Next Generation Optima AUC
- 使用 Multisizer 4e 库尔特颗粒计数及粒度分析仪监测贻贝/软体动物的繁殖
- Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry
- Automated Cell Plating and Growth Assays
- Automated Cell Transfection and Reporter Gene Assay
- Automated Cord Blood Cell Viability and Concentration Measurements Using the Vi‑CELL XR
- Automated Genomic Sample Prep RNAdvance
- Automated salt-assisted liquid-liquid extraction
- Automated Sample Preparation for the Monitoring of Pharmaceutical and Illicit Drugs by LC-MS/MS
- Automated XTT Assay for Cell Viability Analysis
- 自动化生物制药质量控制以降低成本并提高数据完整性
- Automating Bradford Assays
- Automating Cell-Based Processes
- Automating Cell Line Development
- Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity
- The new Avanti J-15 Centrifuge Improves Sample Protection and Maximizes Sample Recovery
- The New Avanti J-15 Centrifuge Time Saving Deceleration Profile Improves Workflow Efficiency
- Avanti JXN Protein Purification Workflow
- Avoid the Pitfalls When Automating Cell Viability Counting for Biopharmaceutical Quality Control
- Beer, Evaluation of Final Product and Filtration Efficiency
- Biomek Automated NGS Solutions Accelerate Genomic Research
- Biomek i-Series Automated IDT® xGen Hybridization Capture of DNA libraries on Biomek i7 Hybrid Genomics Workstation
- Biomek i-Series Automated Illumina® Nextera XT DNA Library Prep Kit
- Biomek i-Series Automated Illumina TruSeq DNA PCR-Free Library Prep Kit
- Biomek i-Series Automated Illumina TruSeq® Nano DNA Library Prep Kit
- Biomek i-Series Automated Illumina TruSeq® Stranded mRNA Sample Preparation Kit Protocol
- Biomek i-Series Automated Illumina TruSeq® Stranded Total RNA Sample Preparation Kit Protocol
- Biomek i–Series Automated Illumina® TruSight Tumor 170 32 Sample Method
- Biomek i-Series Automated KAPA HyperPrep and HyperPlus Workflows
- Biomek i-Series Automated New England Biolabs NEBNext® Ultra IITM DNA Library Prep Kit
- Biomek i-Series Automated Promega Wizard MagneSil Tfx™ Plasmid Purification System
- Biomek i-Series Automated SurePlex PCR and VeriSeq PGS Library Prep for Illumina®
- Biomek i-Series Automation of the Beckman Coulter Agencourt DNAdvance Genomic DNA isolation Kit
- Biomek i-Series Automation of the Beckman Coulter GenFind V3 Blood and Serum DNA Isolation Kit
- Preparation and purification of carbon nanotubes using an ultracentrifuge and automatic dispensing apparatus, and analysis using an analytical centrifuge system
- Cell Counting Performance of Vi–Cell BLU Cell Viability Analyzer
- Cell Culture Monitoring with the Vi-CELL MetaFLEX
- Viability Assessment of Cell Cultures Using the CytoFLEX
- Cell Line Development – Data Handling
- Cell Line Development – Limiting Dilution
- Cell Line Development – Selection and Enrichment
- 库尔特原理分析细胞
- Changes to GMP Force Cleanroom Re-Classifications
- Characterizing Insulin as a Biopharmaceutical Using Analytical Ultracentrifugation
- Classifying a Small Cleanroom using the MET ONE HHPC 6+
- Clean Cabinet Air Particle Evaluation
- Recommended cleaning procedure for the exterior surface of the MET ONE 3400+
- 洁净室常规环境监测 —— FDA 关于 21 CFR Part 11 数据完整性要求
- Comparing Data Quality & Optical Resolution of the Next Generation Optima AUC to the Proven ProteomeLab on a Model Protein System
- 使用MET ONE 3400+ 进行 ISO 14644-3 洁净室自净时间测试
- Considerations of Cell Counting Analysis when using Different Types of Cells
- Consistent Cell Maintenance and Plating through Automation
- Control Standards and Method Recommendations for the LS 13 320 XR
- Counting Efficiency: MET ONE Air Particle Counters and Compliance to ISO-21501
- Critical Particle Size Distribution for Cement using Laser Diffraction
- Use Machine Learning Algorithms to Explore the Potential of Your High Dimensional Flow Cytometry Data Example of a 20–color Panel on CytoFLEX LX
- CytoFLEX
- Detecting and counting bacteria with the CytoFLEX research flow cytometer: II-Characterization of a variety of gram-positive bacteria
- Detecting Moisture in Hydraulic Fluid, Oil and Fuels
- Detection of foreign matter in plating solution using Multisizer4e
- Determination of Size and Concentration of Particles in Oils
- Efficient kit-free nucleic acid isolation uses a combination of precipitation and centrifugation separation methods
- dsDNA Quantification with the Echo 525 Liquid Handler for Miniaturized Reaction Volumes, Reduced Sample Input, and Cost Savings
- Compensation Setup For High Content DURAClone Reagents
- Echo System-Enhanced SMART-Seq v2 for RNA Sequencing
- Efficient Factorial Optimization of Transfection Conditions
- Enhancing Vaccine Development and Production
- Enumeration And Size Distribution Of Yeast Cells In The Brewing Industry
- European Pharmacopoeia EP 2.2.44 and Total Organic Carbon
- Evaluation of Instrument to Instrument Performance of the Vi-CELL BLU Cell Viability Analyzer
- Exosome-Depleted FBS Using Beckman Coulter Centrifugation: The cost-effective, Consistent choice
- Flexible ELISA automation with the Biomek i5 Workstation
- Fully Automated Determination of Benzodiazepines
- Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity
- Get Control in GMP Environments
- Getting Started with Kaluza: Parameters
- g-Max: Added Capabilities to Beckman Coulter's versatile Ultracentrifuge Line
- Grading of nanocellulose using a centrifuge
- A method of grading nanoparticles using ultracentrifugation in order to determine the accurate particle diameter
- Grading of pigment ink and measurement of particle diameter using ultracentrifugation / dynamic light scattering
- HIAC Industrial – Our overview solution for fluid power testing for all applications
- A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System: an application for robust and scalable cancer research and biomarker discovery
- High-Throughput qPCR and RT-qPCR Workflows
- A Highly Consistent BCA Assay on Biomek i-Series
- A Highly Consistent Bradford Assay on Biomek i-Series
- A Highly Consistent Lowry Method on Biomek i-Series
- Highly Reproducible Automated Proteomics Sample Preparation on Biomek i-Series
- Cell Line Development – Hit Picking
- How to Use Violet Laser Side Scatter Detect Nanoparticle
- How Violet Side Scatter Enables Nanoparticle Detection
- HRLD Recommended Volume Setting
- Automating the Cell Line Development Workflow
- ICH Q2 – the Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems
- ICH Q2 – The Challenge of Measuring Total Organic Carbon in Modern Pharmaceutical Water Systems
- Illumina Nextera Flex for Enrichment on the Biomek i7 Hybrid Genomics Workstation
- Importance of TOC measurement in WFI in light of European Pharmacopoeia change
- Improved data quality of plate-based IgG quantification using Spark®’s enhanced optics
- Integration of the Vi-CELL BLU Cell Viability Analyzer into the Sartorius Ambr® 250 High Throughput for automated determination of cell concentration and viability
- Temperature dependence of hydrodynamic radius of an intrinsically disordered protein measured in the Optima AUC analytical ultracentrifuge.
- Issues with Testing Jet Fuels for Contamination
- Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity
- Linearity of BSA Using Absorbance & Interference Optics
- Long Life Lasers
- LS 13 320 XR: Sample Preparation - How to measure success
- Particle Size Analysis Simple, Effective and Precise
- Beckman’s LS 13 320 XR Vs. Malvern Mastersizer
- Using Machine Learning Algorithms to Provide Deep Insights into Cellular Subset Composition
- Flow Cytometric Analysis of auto-fluorescent cells found in the marine demosponge Clathria prolifera
- Matching Cell Counts between Vi–CELL XR and Vi–CELL BLU
- MET ONE Sensor Verification
- Metal colloid purification and concentration using ultracentrifugation
- Separation and purification of metal nanorods using density gradient centrifugation
- Method for Determining Cell Type Parameter Adjustment to Match Legacy Vi CELL XR
- High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler
- Miniaturization and Rapid Processing of TXTL Reactions Using Acoustic Liquid Handling
- Miniaturized Enzymatic Assays with Glycerol
- Miniaturized and High-Throughput Metabolic Stability Assay Enabled by the Echo Liquid Handler
- Miniaturized Multi-Piece DNA Assembly Using the Echo 525 Liquid Handler
- Miniaturized Sequencing Workflows for Microbiome and Metagenomic Studies
- Minimal Sample to Sample Carry Over with the HIAC 8011+
- Minimizing process variability in the manufacturing of bottled drinking water
- Modern Trends in Non‐Viable Particle Monitoring during Aseptic Processing
- Multi-Wavelength Analytical Ultracentrifugation of Human Serum Albumin complexed with Porphyrin
- Particle diameter measurement of a nanoparticle composite - Using density gradient ultracentrifugation and dynamic light scattering
- Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice
- What to do now that ACFTD is discontinued
- Optimizing the HIAC 8011+ Particle Counter for Analyzing Viscous Fluids
- Optimizing the Multisizer 4e Coutler Counter for use with Small Apertures
- Optimizing Workflow Efficiency of Cleanroom Routine Environmental Monitoring
- Particle Counting in Mining Applications
- Particle testing in cleanroom high-pressure gas lines to ISO 14644 made easy with the MET ONE 3400 gas calibrations
- PCR Reaction Setup and AMPure XP Application
- PCR Reaction Setup Application
- Pharma Manufacturing Environmental Monitoring
- Pharma Manufacturing Paperless Monitoring
- Analysis of plant genome sizes using flow cytometry: a case study demonstrating dynamic range and measurement linearity
- Flow Cytometric Approach to Probiotic Cell Counting and Analysis
- Protein purification workflow
- Calibrating the QbD1200 TOC Analyzer
- Detection Limit
- JP SDBS Validation
- USP System Suitability
- 符合《联邦法规 21 章》第 11 部分规定的质量控制电子记录
- Using the Coulter Principle to Quantify Particles in an Electrolytic Solution for Copper Acid Plating
- A Rapid Flow Cytometry Data Analysis Workflow Using Machine Learning- Assisted Analysis to Facilitate Identifying Treatment- Induced Changes
- Rapid Measurement of IgG Using Fluorescence Polarization
- Reducing Errors Associated with NGS Library Preparation
- Root Cause Investigations for Pharmaceutical Water Systems
- Full Automation of the SISCAPA® Workflow using a Biomek NXP Laboratory Automation Workstation
- Specification Comparison of Vi–CELL XR and Vi–CELL BLU
- Specifying Non-Viable Particle Monitoring for Aseptic Processing
- A Standardized, Automated Approach For Exosome Isolation And Characterization Using Beckman Coulter Instrumentation
- Streamlined Synthetic Biology with Acoustic Liquid Handling
- Switching from Oil Testing to Water and back using the HIAC 8011+ and HIAC PODS+
- 使用基于13色管的DURAClone干粉试剂在CytoFLEX流式细胞仪上进行人T细胞亚群的高级分析
- Comparative Performance Analysis of CHO and HEK Cells Using Vi-CELL BLU Analyzer and Roche Cedex® HiRes Analyzer
- Using k-Factor to Compare Rotor Efficiency
- USP 787 Small Volume Testing
- Validation of On-line Total Organic Carbon Analysers for Release Testing Using ICH Q2
- 采用 CytoFLEX 进行囊泡流式细胞术检测
- Vi-CELL BLU FAST Mode Option
- Vi-CELL BLU 符合 21 CFRPart 11的法规要求
- A fully automated plate-based optimization of fed-batch culture conditions for monoclonal antibody-producing CHO cell line
- A High-Throughput, Automated Screening Platform for IgG Quantification During Drug Discovery and Development
- The Valita Aggregation Pure assay: A rapid and accurate alternative for aggregation quantification of purified monoclonal antibodies
- Adaptive Laboratory Evolution of Pseudomonas putida in the RoboLector
- Automated Research Flow Cytometry Workflow Using DURA Innovations Dry Reagent Technology with the *Biomek i7 Automated Workstation and *CytoFLEX LX Flow Cytometer
- Automating antibody titration using a CytoFLEX LX analyzer Integrated with a Biomek i7 Multichannel workstation and Cytobank streamlined data analysis
- Automated IDT Alt-R CRISPR/Cas9 Ribonucleoprotein Lipofection Using the Biomek i7 Hybrid Automated Workstation
- Automation of protein A ELISA Assays using Biomek i7 hybrid workstation
- Monitoring Plant Cell Cultures with BioLector and Multisizer 4e Instruments
- Monitoring E. coli Cultures with the BioLector and Multisizer 4e Instruments
- Monitoring Yeast Cultures with the BioLector and Multisizer 4e instruments
- Biomek i7 Hybrid Automated KAPA mRNA HyperPrep Workflow
- Cluster Count Analysis and Sample Preparation Considerations for the Vi-CELL BLU Cell Viability Analyzer
- Cultivation of suspended plant cells in the BioLector®
- How to use R to rewrite FCS files with different number of channels
- A new approach to nanoscale flow cytometry with the CytoFLEX nano analyzer
- CytoFLEX nano 纳米流式分析仪:纳米级流式细胞仪的前沿新技术
- 利用BioLector进行细胞死亡的测定
- DO-controlled fed-batch cultivation in the RoboLector®
- Screening of yeast-based nutrients for E. coli-based recombinant protein production using the RoboLector Platform
- E. coli fed-batch cultivation using the BioLector® Pro
- Echo System-Enhanced SMART-Seq v4 for RNA Sequencing
- Filling MicroClime Environmental Lids
- Fully Automated Peptide Desalting for Liquid Chromatography–Tandem Mass Spectrometry Analysis Using Beckman Coulter Biomek i7 Hybrid Workstation
- Getting Started with Kaluza: Data Scaling and Compensation Adjustment
- A Simple Guide to Selecting the Right Handheld Particle Counter for Monitoring Controlled Environments
- High throughput cultivation of the cellulolytic fungus Trichoderma reesei in the BioLector®
- High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 525 Liquid Handler
- Host Cell Residual DNA Testing in Reduced Volume qPCR Reactions Using Acoustic Liquid Handling
- Jurkat Cell Analyses Using the Vi-CELL BLU Cell Viability Analyzer
- Linearity of the Vi-CELL BLU Cell Counter and Analyzer
- 利用RoboLector提高谷氨酸棒状杆菌蛋白质产量的培养基优化研究
- MET ONE 3400+ LDAP & Active Directory connection Guide
- 将 CytoFLEX S 流式细胞仪上设计的面板迁移至 CytoFLEX SRTl流式分选仪
- Miniaturization of an Epigenetic AlphaLISA Assay with the Echo Liquid Handler and the BMG LABTECH PHERAstar FS
- Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 555 Liquid Handler
- Miniaturized 16S rRNA Amplicon Sequencing with the Echo 525 Liquid Handler for Metagenomic and Microbiome Studies
- Miniaturized Enzo Life Sciences HDAC1 Fluor de Lys Assays Using an Echo Liquid Handler Integrated in an Access Laboratory Workstation
- Miniaturized EPIgeneous HTRF Assays Using the Echo Liquid Handler
- Miniaturized Gene Expression in as Little as 250 nL
- Miniaturized Genotyping Reactions Using the Echo Liquid Handler
- Mode of operation of optical sensors for dissolved oxygen and pH value
- Nanoliter Scale High-Throughput Protein Crystallography Screening with the Echo Liquid Handler
- Nanoscale Sorting with the CytoFLEX SRT Cell Sorter
- Low-pH profiling in µL-scale to optimize protein production in H. polymorpha using the BioLector
- Optimized NGS Library Preparation with Acoustic Liquid Handling
- Astrios和CytoFLEX SRT流式分选仪的孔板分选速度比较
- Preparation of Mouse Plasma Microsamples for LC-MS/MS Analysis Using the Echo Liquid Handler
- Protocols for use of SuperNova v428 conjugated antibodies in a variety of flow cytometry applications
- Purifying viral vector with VTi 90 rotor and CsCl DGUC
- Robust and High-Throughput SARS-CoV-2 Viral RNA Detection, Research, and Sequencing Using RNAdvance Viral and the OT-2 Platform
- Screening yeast extract to improve biomass production in acetic acid bacteria starter culture
- 用CytoFLEX SRT细胞分选仪进行单细胞分选
- 用CytoFLEX SRT分选稀有E-SLAM造血干细胞及其后续培养
- SWOFF The unrecognized yet indispensable sibling of FMO
- The scattered light signal: Calibration of biomass
- Utilization of the MicroClime Environmental Lid to Reduce Edge Effects in a Cell-based Proliferation Assay
- Vertical Rotor Case Study with Adenovirus
- Variability Analysis of the Vi-CELL BLU Cell Viability Analyzer against 3 Automated Cell Counting Devices and the Manual Method
- Whole Genome Sequencing of Microbial Communities for Scaling Microbiome and Metagenomic Studies Using the Echo 525 Liquid Handler and CosmosID
- Accurate enumeration of phytoplankton using FCM
- Accurately measures fine bubble size and particle count
- Aerobic cultivation of high-oxygen-demanding microorganisms in the BioLector XT microbioreactor
- Anaerobic cultivation processes of probiotic bacteria in the BioLector XT microbioreactor
- Assay Assembly for Miniaturized Quantitative PCR in a 384-well Format Using the Echo Liquid Handler
- Automated Solid Phase Extraction Based Determination of Cannabinoids
- Automated Transfection Methods
- Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex
- Automation of Illumina DNA Prep Kit on Biomek NGeniuS Next Generation Library Prep System
- Biomek基因组样品制备自动化解决方案加速研究进程
- Biomek i-Series Automated AmpliSeq for Illumina® Library Prep Kit
- Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Blood Kit
- Biomek i-Series Automated Beckman Coulter Agencourt RNAdvance Cell
- Biomek i-Series Automated Beckman Coulter Agencourt SPRIselect for DNA Size Selection
- Control of Spheroid Size and Support for Productization
- Data-integrity-and-met-one-3400-plus-function-for-pharma
- Cultivation of Mammalian Cells in the Cydem VT System Bioreactor Module
- Cydem VT Automated Clone Screening System – Generating an Antibody Standard Curve
- Detection of Coarse Particles in Silica Causing Cracks in Semiconductor Encapsulants
- Effective Miniaturization of Illumina Nextera XT Library Prep for Multiplexed Whole Genome Sequencing and Microbiome Applications
- Efficient clone screening with increased process control and integrated cell health and titer measurements with the Cydem VT Automated Clone Screening System
- High-throughput IgG quantitation platform for clone screening during drug discovery and development
- Increased throughput for IgG quantification using Valita Titer 384-well plates
- Introducing the Cydem VT Automated Cell Culture System: A high-throughput platform for fast and reliable clone screening experiments
- CytoFLEX SRT 上的混合模式分选
- Modular DNA Assembly of PIK3CA Using Acoustic Liquid Transfer in Nanoliter Volumes
- Nanoliter Scale DNA Assembly Utilizing the NEBuilder HiFi Cloning Kit with the Echo 525 Liquid Handler
- Performance of the Valita Aggregation Pure assay vs HPLC-SEC
- BioLector XT微型生物反应器小球藻光营养培养
- Precision measurement of adipocyte size with Multisizer4e
- Principles of Continuous Flow Centrifugation
- Purifying High Quality Exosomes using Ultracentrifugation
- Quality Control of Anti-Blocking Powder Particle Size
- Rapid Rabbit IgG Quantification using the Valita Titer Assay
- Leveraging the Vi-CELL MetaFLEX for Monitoring Cell Metabolic Activity
- Unveiling the Hidden Signals: Overcoming Autofluorescence in Spectral Flow Cytometry Analysis
- Unlocking Insights: The Vital Role of Unmixing Algorithms in Spectral Flow Cytometry
- Vaporized Hydrogen Peroxide Decontamination of Vi–CELL BLU Instrument
- Automating the Valita Titer IgG Quantification Assay on a Biomek i-Series Liquid Handling System
- Viral Vector Purification with Ultracentrifugation
- Analytical Ultracentrifugation (AUC) for Characterization of Lipid Nanoparticles (LNPs): A Comprehensive Review
- Leveraging Analytical Ultracentrifugation for Comprehensive Characterization of Lipid Nanoparticles in Drug Delivery Systems
-
彩页
- Access Single Robot System——合成生物学工作流利器
- Automated Solutions for Cell Line Development
- Automated Solutions for ELISA
- Echo Acoustic Liquid Handling for Synthetic Biology
- HIAC 8011+ Liquid Particle Counting Systems
- HIAC 9703+ Sub-Visible Particulate Testing
- LS 13 320 XR - Laser Diffraction Particle Size Analyzer
- ValitaTiter IgG定量试剂盒彩页下载
-
案例分析
- Adenoviral Vectors Preparation
- Algae Biofuel Production
- Antibody and Media Development
- Autophagy
- B Cell Research
- Basic Research on Reproductive Biology
- Cardiovascular Disease Research
- Cell Marker Analysis
- Choosing a Tabletop Centrifuge
- Collagen Disease Treatment
- Controlling Immune Response
- Creating Therapeutic Agents
- DNA Extraction from FFPE Tissue
- English Safety Seminar
- Equipment Management
- Exosome Purification Separation
- Fast, Cost-Effective and High-Throughput Solutions for DNA Assembly
- Future of Fishing Immune Research
- Hematopoietic Tumor Cells
- High-throughput next-generation DNA sequencing of SARS-CoV-2 enabled by the Echo 525 Liquid Handler
- Hiroshima Genbaku HP Hematopoietic Tumor Testing
- iPS Cell Research
- Leveraging acoustic and tip-based liquid handling to increase throughput of SARS-CoV-2 genome sequencing
- Membrane Protein Purification X Ray Crystallography
- Organelles Simple Fractionation
- Particle Interaction
- Quality evaluation of gene therapy vector
- Retinal Cell Regeneration
- Sedimentary Geology
- Severe Liver Disease Treatment
- Tierra Biosciences reveals major molecular discovery
- Treating Cirrhosis
- University Equipment Management
- Fundamentals of Ultracentrifugal Virus Purification
- 产品目录
- 单页
-
专家访谈
- Background and Current Status of the Introduction of Flow Cytometers
- Benefits-of-the-coulter-principle-in-the-manufacturing-for-ips-cell-derived-natural-killer-cells
- Central Diagnosis in the Treatment of Childhood Leukemia 1
- Central Diagnosis in the Treatment of Childhood Leukemia 2
- Challenges-in-viability-cell-counting
- Contribution of Cytobank to 1-cell analysis of the cancer microenvironment
- Development of technology for social implementation of synthetic biology
- Flow Cytometry Testing in Hospital Laboratories
- Fundamentals of Ultracentrifugal Virus Purification
- The MET ONE 3400+ Automates Routine Environmental Monitoring for GMP Cleanroom Compliance
- Tumor Suppressor Gene p53 research and DNA Cleanup Process
- Fundamentals of Ultracentrifugal Virus Purification
- Dr Yabui UCF Lecture
-
主题报告
- Applications of Ultracentrifugation in Purification and Characterization of Biomolecules
- Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation
- ABRF 2019: Automated Genomic DNA Extraction from Large Volume Whole Blood
- Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation
- Automating Cell Line Development for Biologics
- Cellular Challenges: Taking an Aim at Cancer
- Cell-Line Engineering
- Characterizing the Light-Scatter Sensitivity of the CytoFLEX Flow Cytometer
- AACR 2019: Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample
- Mastering Cell Counting
- Preparing a CytoFLEX for Nanoscale Flow Cytometry
- A Prototype CytoFLEX for High-Sensitivity, Multiparametric Nanoparticle Analysis
- ABRF 2019: Simultaneous DNA and RNA Extraction from Formalin-Fixed Paraffin Embedded (FFPE) Tissue
- Quantification of AAV Capsid Loading Fractions: A Comparative Study
- Using Standardized Dry Antibody Panels for Flow Cytometry in Response to SARS-CoV2 Infection
- 产品说明书
- 实验步骤
-
白皮书
- Centrifugation is a complete workflow solution for protein purification and protein aggregation quantification
- AUC Insights - Analysis of Protein-Protein-Interactions by Analytical Ultracentrifugation
- A General Guide to Lipid Nanoparticles
- Addressing issues in purification and QC of Viral Vectors
- GMP Cleanrooms Classification and Routine Environmental Monitoring
- Purification of Biomolecules by DGUC
- AUC Insights - Assessing the quality of adeno-associated virus gene therapy vectors by sedimentation velocity analysis
- AUC Insights - Sample concentration in the Analytical Ultracentrifuge AUC and the relevance of AUC data for the mass of complexes, aggregation content and association constants
- Analyzing Biological Systems with Flow Cytometry
- 亚可见颗粒物检测新进展:USP <1788>的最新修订
- Changes to USP <643> Total Organic Carbon
- Characterization of RNAdvance Viral XP RNA Extraction Kit using AccuPlex™ SARS–CoV–2 Reference Material Kit
- CytoFLEX Platform Flow Cytometers with IR Laser Configurations: Considerations for Red Emitting Dyes
- Evaluation of the Analytical Performance of the AQUIOS CL Flow Cytometer in a Multi-Center Study
- Simultaneous Isolation and Parallel Analysis of gDNA and total RNA for Gene Therapy
- Hydraulic Particle Counter Sample Preparation
- Inactivation of COVID–19 Disease Virus SARS–CoV–2 with Beckman Coulter Viral RNA Extraction Lysis Buffers
- Tips for Cell Sorting
- IVD-R Annex I Global Safety and Performances Requirements
- Liquid Biopsy Cancer Biomarkers – Current Status, Future Directions
- MET ONE 3400+ IT Implementation Guide
- Reproducibility in Flow Cytometry
- SuperNova v428: New Bright Polymer Dye for Flow Cytometry
- SuperNova v428: New Bright Polymer Dye for Flow Cytometry
- Japan Document
-
应用手册